The Intracellular Cryoprotectant Effects in Preserving Goramy Spermatozoa after Two Days Sub-Zero Freezing

Effects of In Vitro Zinc Sulphate Additive to The Semen Extender on Water Buffalo (Bubalusbubalis) Spermatozoa before and after Freezing

Lower intracellular concentration of cryoprotectants after vitrification than after slow freezing despite exposure to higher concentration of cryoprotectant solutions.

STUDY QUESTION What is the intracellular concentration of cryoprotectant (ICCP) in mouse zygotes during vitrification (VIT) and slow-freezing (SLF) cryopreservation procedures? SUMMARY ANSWER Contrary to common beliefs, it was observed that the ICCP in vitrified zygotes is lower than after SLF, although the solutions used in VIT contain higher concentrations of cryoprotectants (CPs). WHAT I...

Effects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (Bubalus bubalis)

The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ˚C with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg mL-1 sodium selenite and the sperm motility and...

The Efficacy of Glucose and Electrolyte Solutions in Preserving the Structure of Fowl Spermatozoa after Freezing and Thawing

HARRIS et al.1), observing ultrastructural changes in rapidly frozen fowl spermatozoa, noted destructive effects on the acrosome, the midpiece, and the cytoplasmic membrane. Similar ultrastructural damages were seen by BAKST and SEXTON2) in frozen-thawed fowl and turkey spermatozoa under transmission and scanning electron microscopes. However, they gave little information on the possible mechan...

Comparison of Two Cryopreservation Protocols for Freezing Human Spermatozoa

FREEZING HUMAN SPERMATOZOA Hiroshi Kobayashi, Pavithra Ranganathan, Ayman Mahran, Rakesh Sharma, Anthony J. Thomas, Ashok Agarwal, The Cleveland Clinic Foundation Objective: Sperm quality decreases significantly following freezing and research on improving cryosurvival rates is crucial. We compared the effects of two cryopreservation protocols to determine which method allows better preservatio...